Ras-associated, small GTP-binding protein
rat, recombinant, E. coli
For in vitro use only!
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 54 kDa
Accession number: P23640
Purity: > 90 % (SDS-PAGE)
Form: liquid (Supplied in 25 mM HEPES pH 7.2, 40 mM NaCl, 2 mM MgCl2, 10 μM GDP, 3 mM DTE and 10% glycerol)
Activity: 100 pmol of protein can bind > 80 pmol of GDP.
N-terminal tagged Rab27 is a small GTPase that belongs to the Ras superfamily. Rab proteins play an important role in various aspects of membrane traffic, including cargo selection, vesicle budding, vesicle motility, tethering, docking, and fusion. The monomeric GTPase Rab27 subfamily regulates the exocytosis of these cell-specific store organelles. Rab27 acts through organelle-specific effector proteins, such as granuphilin in pancreatic beta cells and melanophilin in melanocytes. The Rab27 and effector complex then interacts with proteins that are essential for membrane transport and fusion. The GST-Tag facilitates the protein`s application in typical GST pull-down assays.
BIOZ Product Citations:
Please click the arrow on the right to expand the citation list. Click publication title for the full text.
Stenmark et al. (2001) The Rab GTPase family. Genome Biol. 2:30071.
Somsel et al. (2000) Rab GTPases coordinate endocytosis. J. Cell Sci. 113:183.
Izumi et al. (2003) The roles of Rab27 and its effectors in the regulated secretory pathways. Cell Struct. Funct. 28:465.