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GGTase-IF53Y_Y126TGST

Protein geranylgeranyltransferase type I mutant, α- and β-subunit

rat, recombinant, E. coli

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GGTase-IF53Y_Y126TGST PR-954 20 μg 281,75 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on dry ice

Storage Conditions: store at -80 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: GST-α: 73 kDa, β: 44 kDa

Purity: > 90 % (SDS-PAGE)

Form: liquid (Supplied in 25 mM HEPES pH 7.2, 40 mM NaCl and 2 mM DTT)

pH: 7.2

Activity: 1 pmol of GGTase-I double mutant will transfer 0.5 pmol of BGPP to RhoA in 15 min at 37°C.

Description:
GGTase-I is one of the three mammalian protein prenyltransferases. The double mutant of GGTase-I was engineered to efficiently transfer biotin-geranylpyrophosphate (BGPP, Cat.# LI-015) onto its native protein substrates. This approach allows the detection of femtomolar amounts of prenylatable proteins in eukaryotic cells and tissues as well as effective inhibitor screening.

Selected References:
Hougland et al. (2009) Getting a handle on protein prenylation. Nat. Chem. Biol. 5 (4):197.
Nguyen et al. (2009) Analysis of the eukaryotic prenylome by isoprenoid affinity tagging. Nat. Chem. Biol. 5 (4):227