REP-1 ^His

For general laboratory use.

Shipping: shipped on dry ice

Storage Conditions: store at -80 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 75 kDa

Purity: > 90 % (SDS-PAGE)

Form: liquid (Supplied in 25 mM HEPES pH 7.2, 40 mM NaCl and 5 mM DTT)

Activity: 1 pmol of REP-1 will mediate transfer of 2 pmol of geranylgeraniol by RabGGTase onto 1 pmol of Rab7 in 3 minutes.

Description:
C-terminal His-tagged REP-1 (Rab escort protein-1) binds to a wide range of RabGGTase with nanomolar affinities and supports the prenylation of RabGTPase mediated by RabGGTase.

BIOZ Product Citations:

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Selected References:
Seabra et al. (1995) Deficient geranylgeranylation of Ram/Rab27 in choroideremia. J. Biol. Chem. 270:24420.
Alexandrov et al. (1994) Rab escort protein-1 is a multifunctional protein that accompanies newly prenylated rab proteins to their target membranes. EMBO J. 13:5262.
Watanabe et al. (2008) Inhibitors of Protein Geranylgeranyltransferase I and Rab Geranylgeranyltransferase Identified from a Library of Allenoate-derived Compounds. J. Biol. Chem. 283 (15):9571-9579.