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GsαS-Leu212-Asn280

stimulatory heterotrimeric G-protein, short splice variant of the α-subunit

rat, recombinant, Sf9 insect cells

Product Cat. No. Amount Price (EUR) Buy / Note
GsαS-Leu212-Asn280 PR-506 1 ml 345,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on dry ice

Storage Conditions: store at -80 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 45 kDa

Accession number: NM_019132

Accession number: NM_019132

Form: Membrane suspension (Supplied in 75 mM Tris-HCl pH 7.4, 12.5 mM MgCl2 and 1 mM EDTA)

pH: 7.4

Description:
GsαS is the short splice variant of the α-subunit of stimulatory heterotrimeric Gs-proteins. In contrast to the long splice variant (GsαL) GsαS lacks the 15-amino acid insert between the Ras like and the α-helical domain. GsαS activates adenylate cyclase (AC) and possesses a higher GDP-affinity than GsαL (cat.# PR-501). The differences in GDP-binding between GsαS and GsαL have important consequences for receptor/G-protein coupling and activation. In contrast to all other known Gα D/N mutants, the exchange of Asp280 to Asn280 in GsαS does not lead to an inactivation in nucleotide binding. Mutation of Gln212 to Leu212 inhibits the intrinsic GTPase activity, resulting in a constitutively activated GsαS. This mutation also increases the GDP-affinity of GsαS.

Selected References:
Graziano et al. (1989) Expression of G in Escherichia coli. Purification and properties of two forms of the protein. J. Biol. Chem. 264:409.
Yu et al. (1998) Interaction of the Xanthine Nucleotide Binding G Mutant with G Protein-coupled Receptors. J. Biol. Chem. 273:30183.
Gille et al. (2003) 2' (3')-O- (N-Methylanthraniloyl)- substituted GTP Analogs: A Novel Class of Potent Competitive Adenylyl Cyclase Inhibitors. J. Biol. Chem. 278:12672.
Gille et al. (2003) GDP Affinity and Order State of the catalytic Site Are Critical for Function of Xanthine Nucleotide-selective Gαs Proteins. J. Biol. Chem. 278:7822.