Rheb

For general laboratory use.

Shipping: shipped on dry ice

Storage Conditions: store at -80 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 47 kDa

Accession number: NM_005614

Purity: > 90 % (SDS-PAGE)

Form: liquid (Supplied in 25 mM HEPES pH 7.4, 5 mM MgCl₂ and 10 μM GTP)

Description:
Recombinant human Rheb expressed in E. coli as fusion protein with GST. The eukaryotic translational machinery is under tight control by environmental and metabolic cues including ambient nutrients, hormonal regulation and the energy status of the cell itself (1). The Ras-related small GTPase Rheb (Ras homologue enriched in brain) acts as a pivotal player upstream of mTOR and p70S6K to regulate global as well as selective protein translation in response to the above mentioned factors (2,3).

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Selected References:
Arsham et al. (2006) Thinking globally and acting locally with TOR. Curr Opin Cell Biol 18:589.
Garami et al. (2003) Insulin Activation of Rheb, a Mediator of mTOR/S6K/4E-BP Signaling, is Inhibited by TSC1 and 2. Cell 11:1457.
Roux et al. (2004) Tumor-promoting phorbol esters and activated Ras inactivate the tuberous sclerosis tumor suppressor complex via p90 ribosomal S6 kinase. Proc. Natl. Acad. Sci. USA 101:13489.