For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Formula: KKISGRLSPIMTEQ
Molecular Weight: 1588 g/mol
Purity: 90 - 95 % (HPLC)
Form: lyophilized (reconstitution in ddH2O)
The synthetic peptide KKISGRLSPIMTEQ can be used as a substrate for DYRK family kinases in in vitro kinase assays. The peptide corresponds to residues 324-334 of transcription factor FKHR with two lysine residues added at the N-terminus to facilitate binding to phosphocellulose paper.
DYRK2, a dual-specificity tyrosine-(Y)-phosphorylation regulated kinase gene, demonstrate the highest mRNA overexpression level among the genes tested in gastroesophageal and lung adenocarcinomas.
Protein kinase(s) from the DYRK family (dual specificity tyrosine phosphorylated and regulated kinase) may be involved in a new mechanism for the regulation of glycogen synthesis.
Woods et al. (2001) The kinase DYRK1A phosphorylates the transcription factor FKHR at Ser329 in vitro, a novel in vivo phosphorylation site. Biochem. J. 355:597.
Miller et al. (2003) Amplification and overexpression of the dual-specificity tyrosine (Y) phosphorylation regulated kinase 2 (DYRK2) gene in esophageal and adenocarcinomas. Cancer Res. 63:4136.
Skurat et al. (2004) Phosphorylation of Ser640 in muscle glycogen synthase by DYRK family protein kinases. J. Biol. Chem. 279:2490.