For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Formula: RRRDDDSDDD
Molecular Weight: 1264 g/mol
Purity: 90 - 95 % (HPLC)
Form: lyophilized (reconstitution in ddH2O)
The peptide RRRDDDSDDD can be used as a substrate for CK2 (casein kinase 2) in in vitro kinase assays. It is phosphorylated by CK2 with a Km of 50 μM and a Vmax of 159.7 nmol/min/mg.
Casein kinase 2 (CK2) is a ubiquitous pleiotropic proliferation-associated serine/threonine protein kinase. Similar to protein kinase A, CK2 is a tetramer made up from two different subunits a2b2. The catalytic a-subunit corresponds to the C-subunit of PKA, the non-catalytic b-subunit is unique and differs from the R-subunit of PKA in all known features.
Recently it was shown that the b subunit is trifunctional: (i) it confers stability to the holoenzyme, (ii) it increases enzyme activity, and (iii) it determines substrate specificity.
Meggio et al. (1994) Substrate specificity of protein kinase CK2. Cell. Mol. Biol. Res. 40:401.
Grankowski et al. (1991) Isolation and characterization of recombinant human casein kinase II subunits alpha and beta from bacteria. Eur. J. Biochem. 198:25.
Meggio et al. (1992) Role of the beta subunit of casein kinase-2 on the stability and specificity of the recombinant reconstituted holoenzyme. Eur. J. Biochem. 204:293.
Issinger et al. (1992) Characterization of the alpha and beta subunits of casein kinase 2 by far-UV CD spectroscopy. Biochemistry 31:6098.