For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Formula: KKKVSRSGLYRSPSMPENLNRPR
Molecular Weight: 2701 g/mol
Purity: 90 - 95 % (HPLC)
Form: lyophilized (reconstitution in 1.85 ml ddH2O results in a 200 μM solution)
The synthetic peptide KKKVSRSGLYRSPSMPEN LNRPR can be used as a substrate for CHK (checkpoint kinase) 1and 2 in in vitro kinase assays. Its sequence is derived from cdc25C.
Checkpoint kinase 1 (CHK1) blocks the activation of the Cdc2-cyclin B kinase complex, and hence entry into mitosis, by disrupting the translocation of the phosphatase Cdc25C from the cyotoplasm to the nucleus.
Checkpoint kinase 2 (CHK2) is emerging as a key mediator of diverse cellular responses to genotoxic stress, guarding the integrity of the genome throughout eukaryotic evolution. Recent studies show the fundamental role of CHK2 in the network of genome-surveillance pathways that coordinate cell-cycle progression with DNA repair and cell survival or death. Defects in CHK2 contribute to the development of both hereditary and sporadic human cancers, and earmark this kinase as a candidate tumour suppressor and an attractive target for drug discovery.
O'Neill et al. (2002) Determination of substrate motifs for human Chk1 and hCds1/Chk2 by the oriented peptide library approach. J. Biol. Chem. 277:16102.
Bartek et al. (2001) CHK2 kinase--a busy messenger. Nat. Rev. Mol. Cell Biol. 12:877.
Gire et al. (2004) DNA damage checkpoint kinase Chk2 triggers replicative senescence. EMBO J. 23:2554.
Li et al. (2002) Targeting serine/threonine protein kinase B/Akt and cell-cycle checkpoint kinases for treating cancer. Curr. Top. Med. Chem. 2:939.