anti-bovine prion protein
murine, monoclonal, clone 3C10
For in vitro use only!
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Form: liquid (Supplied in culture medium supplemented with FBS)
mAb 3C10 may be used for Western blotting and ELISA at a dilution of 1:5000 - 1:20000.
Monoclonal antibody (mAb)3C10 is a murine IgG2a subtype antibody. mAb 3C10 was produced in a Tecnomouse hollow fibre in vitro cell cultivation system from Integra Biosciences AG, Switzerland. In the production medium, fetal bovine serum components, phenol red and basic nutrients are still present. The antibody is supplied with 0.01 % thimerosal as preservative.
mAb 3C10 recognizes the sequence G108QWNKP113 of the bovine prion protein (human PrP: amino acids 97-102). This sequence near the N-terminal proteinase K digestion site is conserved to some extent in known mammalian prion proteins. mAb 3C10 detects recombinant PrPc from cattle, sheep, hamster, mouse and human in Western blots. It also detects the bovine prion protein in immunoblots of brain homogenates of healthy and BSE-affected animals, and the partly refolded recombinant bovine PrP in ELISA applications.
PrP from brain homogenates of BSE-infected cattle (lanes 1 and 4) appears with three glycosylation-dependent bands, with masses between 16 and 36 kDa, on Western blots. Treatment of the homogenates with proteinase K under defined conditions degrades the abundant PrPC molecules, revealing the shortened PrPSc (lanes 2 and 5). Proteinase K cleaves some residues from PrPSc, causing an apparent decrease in its molecular mass. Recombinant bovine PrPC runs at 27 kDa, the dimer at 48 kDa (lanes 3 and 6).
Species and prion protein sequence:
Human, Chimpanzee, Goat, Sheep, Pig: SQWNKP
Cattle, Horse: GQWNKP
Hamster, Mouse, Rat: NQWNKP
Dog, Mink, Ferret: GQWGKP
Schaller et al. (1999) Validation of a western immunoblotting procedure for bovine PrPSc detection and its use as a rapid surveillance method for the diagnosis of bovine spongiform encephalopathy (BSE). Acta Neuropathologica 98:437.
Kübler et al. (2003) Diagnosis of prion diseases. Br. Med. Bull. 66:267.
Kim et al. (2004) Antigenic characterization of an abnormal isoform of prion protein using a new diverse panel of monoclonal antibodies. Virology 320:40.