anti human Methionine Sulfoxide Reductase B3
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Form: liquid (Supplied as serum, preserved in glycerol (50 % saturation))
Anti-human-MSRB3 (h-Methionine sulfoxide reductase B3) is a rabbit polyclonal antibody that was produced against the recombinant human MsrB3. Human methionine sulfoxide reductase B3 (hMsrB3) is an endoplasmic reticulum (ER) reductase that catalyzes the stereospecific reduction of methionine-R-sulfoxide to methionine in proteins. MSRB3-catalyzed reduction of methionine sulfoxides to methionine is essential for hearing.
Anti-human MsrA can be used for Western blot, ELISA and Immunocytochemistry.
Antibody specificity was evaluated by indirect immunofluorescence and western blotting. Western blot of 50 μg protein of the human oral mucosa, parathyroid and thyroid gland revealed in each case the protein band of the 23 kDa MsrB3. Anti-MSRB3 is without crossreactivity to other human proteins or MSRs (MSRA, MSRB1 and MSRB2).
The antibody visualized the MSRB3- immunoreactivity into the cytoplasma of parathyroid cells (unpublished result, Fig. Merged the MSRB3-immunoreactivity (Cy-3, red) with DAPI staining of the nuclei (blue).
Ahmed et al. (2011) Functional null mutations of MSRB3 encoding methionine sulfoxide reductase are associated with human deafness DFNB74. Am. J. Hum. Genet. 88 (1):19.