For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at 4 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Form: liquid (Supplied as ascites fluid) For purification from ascites fluid, Mab-OA1 was precipitated twice with ammonium sulfate. The antibody is preserved in ammonium sulfate (50% saturation).
Monoclonal Antibody to octopamine (mAb-OA1) is a mouse IgG2a subclass antibody.
Mab OA-1 can be used for immunocytochemistry and ELISA (1:1000).
To establish the specificity of Mab OA-1, we developed a highly sensitive indirect competitive ELISA. This qualitative and quantitative screening revealed that Mab OA-1 had high affinity to the tested octopamine-poly-L-lysine conjugate. Seven different biogenic amines ranging in concentration from 0,39-400 μg/ml were tested for a possible inhibition to the high-affinity reaction. The relative cross-reactivity was: 100% to octopamine; 63% to epinephrine; 21% to tyramine; 8% to norepinephrine; Dopamine, DOPA, and serotonine showed 0% inhibition.
The antibody is binding to synthetic octopamine conjugates in a indirect competitive ELISA procedure and native octopamine in the immunocytochemistry of nervous system of invertebrates.
Make sure to mix it well before use! The antibody is within the precipitate.
Dacks et al. (2005) Octopamine-Immunoreactive Neurons in the Brain and Subesophageal Ganglion of the Hawkmoth Manduca sexta. J Comp Neurol 88:255.
Kononenko et al. (2009) Tyramine as an independent transmitter and a precursor of octopamine in the locust central nervous system: an immunocytochemical study. The Journal of comparative neurology. 512 (4):433.
Cholewa et al. (2009) Descending unpaired median neurons with bilaterally symmetrical axons in the suboesophagealganglionof Manduca sexta larvae. Zoology 112 (4):251.
Rigby et al. (2011) Roles of biogenic amines in regulating bioluminescence in the Australian glowworm Arachnocampa flava. The Journal of Experimental Biology 214 (19):3286.