5'-Biotin-ApG
For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C36H53N13O16P2S (free acid)
Molecular Weight: 1017.90 g/mol (free acid)
Exact Mass: 1017.29 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: filtered solution (30 kDa) in water
Color: colorless to slightly yellow
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 260 nm, ε 25.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Initiator dinucleotide for the 5'-labeling of RNA by in vitro transcription[1]
Selected References:
[1] Pitulle et al. (1992) Initiator oligonucleotides for the combination of chemical and enzymatic RNA synthesis. Gene. 112 (1):101.
| Cat. No. | Amount | Price |
|---|---|---|
| NU-888-BIO-S | 10 μl (100 mM) | 335,10 € |
| NU-888-BIO-L | 5 x 10 μl (100 mM) | 1.340,20 € |
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Scientific citations for Jena Bioscience nu-888-bio
1 scientific citations and 0 citation images found by Bioz.
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Establishment of 5′–3′ interactions in mRNA independent of a continuous ribose-phosphate backbone
RNA, 2020-05-01 · PMID:32111664
.. a 5′ biotin-ApG initiator nucleotide was incorporated cotranscriptionally ( ).. A 25 μL transcription reaction contained 1x transcription buffer (Ambion), 30 mM DTT, 10 mM MgCl 2 , 0.01 U pyrophosphatase (Thermo Fisher), 20 U RNasin (Promega), 1 μg linearized plasmid,
1.5 mM 5′ biotin-ApG (Jena Bioscience ), 1.5 mM ATP and CTP, 1 mM UTP, 20 μCi α- 32 P UTP, and 100 U T3 RNA polymerase (NEB).. Reactions were started by addition of 0.15 mM GTP.Reactions were ..
