» Sign in / Register

NHS Esters of Biotin

NHS Ester of Biotin enable Biotin labeling of proteins (e.g. antibodies) and any other primary amine-containing macromolecules. These biotinylated molecules are subsequently detected via fluoresecent or HRP-labeled streptavidin.

Binding of Biotin by avidin, streptavidin or NeutrAvidinTM is the strongest known biological interaction with a dissociation constant in the range of 10-15 M. The vitamin Biotin may be conjugated to many proteins without loss of their biological activity due to the small size of the biotin molecule.