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Diazo Biotin-DBCO

Cat. No. Amount Price (EUR) Buy / Note
CLK-1043-5 5 mg 214,50 Add to Basket/Quote Add to Notepad
CLK-1043-25 25 mg 943,20 Add to Basket/Quote Add to Notepad
Structural formula of Diazo Biotin-DBCO
Structural formula of Diazo Biotin-DBCO

For research use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C

Shelf Life: 12 months after date of delivery

Molecular Formula: C52H60N8O9S

Molecular Weight: 973.15 g/mol

Purity: > 95 % (HPLC)

Form: solid

Color: orange

Solubility: DMSO, DMF

Diazo Biotin-DBCO is suitable for the introduction of a biotin moiety to Azide-labeled biomolecules via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction.

The biotinylated molecule can be selectively captured through conventional affinity biotin - streptavidin purification and released under mild buffer conditions with a sodium dithionite (Na2S2O4) solution for subsequent identification by mass spectrometry, ELISA, dot blot or Western blot applications.

Important Product Information
If possible, avoid reducing agents in reaction buffers, this can interfere with the azide stability.

General Procedure for Release
Incubate beads in phosphate buffer solution (pH 7.4) containing 50 mM Na2S2O4 (2 x 60 minutes) at room temperature. References [1]-[3] might be used as starting point for the set up of your individual assay.

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Selected References:
[1] Szychowski et al. (2010). Cleavable Biotin Probes for Labeling of Biomolecules via Azide-Alkyne Cycloaddition. J. Am. Chem. Soc. 132 (51):18351.
[2] Verhelst et al. (2007). A Mild Chemically Cleavable Linker System for Functional Proteomic Applications. Anweg. Chem. Int. Ed. 46 (8):46 (8):1284.
[3] Yang et al. (2010). Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry. J. Am. Chem. Soc. 132 (11):3640.