For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Shelf Life: 12 months after date of delivery
Molecular Formula: C33H45N9O7S
Molecular Weight: 711.83 g/mol
Purity: > 95 % (HPLC)
Color: orange to grey
Solubility: DMSO, DMF
Diazo Biotin-Azide is suitable for the introduction of a biotin moiety to terminal Alkyne- and strained Alkyne (e.g. DBCO)-labeled biomolecules via Cu(I)-catalyzed Alkyne-Azide (CUAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction, respectively.
The biotinylated molecule can be selectively captured through conventional affinity biotin - streptavidin purification and released under mild buffer conditions with a 50 mM sodium dithionite (Na2S2O4) solution for subsequent identification by mass spectrometry, ELISA, dot blot or Western blot applications.
Important Product Information
If possible, avoid reducing agents in reaction buffers, this can interfere with the azide stability.
General Procedure for Release
Incubate beads in phosphate buffer solution (pH 7.4) containing 50 mM Na2S2O4 (2 x 60 minutes) at room temperature. References - might be used as starting point for the set up of your individual assay.
 Szychowski et al. (2010). Cleavable Biotin Probes for Labeling of Biomolecules via Azide-Alkyne Cycloaddition. J. Am. Chem. Soc. 132 (51):18351.
 Verhelst et al. (2007). A Mild Chemically Cleavable Linker System for Functional Proteomic Applications. Anweg. Chem. Int. Ed. 46 (8):46 (8):1284.
 Yang et al. (2010). Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry. J. Am. Chem. Soc. 132 (11):3640.