» Sign in / Register



Cat. No. Amount Price (EUR) Buy / Note
CLK-A128-25 25 mg 141,34 Add to Basket/Quote Add to Notepad
CLK-A128-100 100 mg 364,50 Add to Basket/Quote Add to Notepad
CLK-A128-1000 1 g 1.926,62 Add to Basket/Quote Add to Notepad
Structural formula of DBCO-PEG4-DBCO (Dibenzylcyclooctyne-PEG4-Dibenzylcyclooctyne)
Structural formula of DBCO-PEG4-DBCO

For research use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C

Shelf Life: 12 months after date of delivery

Molecular Formula: C50H54N4O9

Molecular Weight: 854.99 g/mol

Purity: ≥ 90 % (HPLC)

Form: amorphous solid to sticky semisolid

Solubility: DCM, DMF, DMSO, THF

Conversion of azide group into DBCO group
Post-synthetic modification of azido-containing peptides
Peptide-peptide conjugation
Surface modification

DBCO-PEG4-DBCO is a reagent for converting of azido-modified peptides or biopolymers into DBCO-modified peptides or biopolymers. When applied in stoichiometric amount DBCO-PEG4-DBCO can serve as a homofunctional azide-to-azide crosslinker for covalently linking azido-modified peptides or biopolymers via a stable triazole moiety. Once a peptide or biopolymer is DBCO-labeled, it can be reacted with an azide-labeled molecule to produce a stable conjugate. The DBCO group reacts with an azide to produce a stable triazole, which is also referred to as the Cu(I)-free or strain-promoted click reaction.

Procedure for Conversion Reaction for Small Molecules

  • Prepare the azide-containing sample in reaction buffer or organic solvent.
  • Dissolve DBCO-PEG4-DBCO in organic solvent. Water-miscible solvent (e.g. DMSO or DMF) should be used if azido-containing sample is in aqueous buffer.
  • Add azido-containing sample to 5 - 10 fold excess DBCO-PEG4-DBCO.
  • Note: Reverse addition order will result in formation of substantial amount of crossliked by-product.
  • Incubate the reaction at room temperature for 2 - 4 hours or at 4 °C for 2 - 12 hours.
  • The reaction is now ready for purification.


Problem: large amount of crosslinked product if formed

  • Possible reason: excess of DBCO-PEG4-DBCO is not large enough
    - Increase molar excess of DBCO-PEG4-DBCO
    - Lower reaction temperature

Product Citations:
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.