5'-Azido-puromycin
For research use only.
Description:
Gee et al.[1] reported a non-radioactive alternative to analyze newly synthesized proteins in cell culture that is based on 5'-Azido-puromycin, an azide analog of puromycin.
5'-Azido-puromycin is cell-permeable and incorporates into the C-terminus of translating polypeptide chains thereby stopping translation.
The resulting truncated C-terminal azide-labeled proteins can subsequently be detected via copper-free click chemistry that offers the choice to introduce a Biotin group (via DBCO-modified Biotin) for subsequent purification tasks or a fluorescent group (via DBCO-modified fluorescent dyes) for subsequent microscopic imaging.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C22H28N10O4 (free amine)
Molecular Weight: 496.53 g/mol (free amine)
Exact Mass: 496.23 g/mol (free amine)
Purity: ≥ 95 % (HPLC)
Form: solid
Color: colorless
Solubility: DMSO, PBS (up to 50 mM tested) pH adjusted to 5.0
Spectroscopic Properties: λ 268 nm, ε 19.5 L mmol-1 cm-1 (0.1 M HCl)
Selected References:
Gee et al. (2016) Puromycin Analogues Capable of Multiplexed Imaging and Profiling of Protein Synthesis and Dynamics in Live Cells and Neurons. Angew. Chem. Int.Ed.55: 4933.
| Cat. No. | Amount | Price |
|---|---|---|
| CLK-110-L | 10 x 0,5 mg (10 μmol) | 766,40 € |
| CLK-110-S | 0,5 mg (1 μmol) | 146,36 € |
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Scientific citations for Jena Bioscience clk-110
2 scientific citations and 1 citation images found by Bioz.
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Life without cytoplasm: sperm sustain protein production utilizing cytoplasmic droplets and mitochondria as translational apparatuses
bioRxiv, 2026-08-11 · DOI:10.64898/2026.08.10.744062
PrePrint: .. cauda sperm were divided into 5′-azido-puromycin (AP)-treated and untreated groups.. The AP group was incubated with
10 μM 5′-azido-puromycin (Cat#CLK-110 -S,Jena Bioscience ) in EKRB at 34°C for 30 min; control sperm were cultured in EKRB alone at 34°C for 30 min. After two PBS washes, samples were lysed, sonicated, and acetone-precipitated as described above.. Pre-cleared supernatants (endogenous biotin removal with streptavidin beads) were reacted .. -
Dermokine mutations contribute to epithelial-mesenchymal transition and advanced melanoma through ERK/MAPK pathways
Europe PMC, 2022-10-28 · DOI:
.. lentivirus-containing medium in the presence of 8 μg/ml polybrene according to the manufacturer's instruction.. Following the 12-hour incubation, infected cells were selected by adding
2 g/ml Puromycin (Jena Bioscience , Jena, Germany) until no more living cells were present in the control plate (untransduced).. The transfection e ciency of the expanded single-cell clone was con rmed by using ..
