Streptavidin, Alexa Fluor® 488 Conjugate
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Shelf Life: 12 months after date of delivery
Form: yellow to orange solid containing lyophilized PBS buffer (pH 7.2) and 0.1% BSA (without sodum azide)
Spectroscopic Properties: λexc 499 nm, λem 520 nm, ε 73.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
AF488 Streptavidin Conjugate (also known as Alexa Fluor® 488 Streptavidin Conjugate) is ideally suited for the specific, fluorescence-based detection of biotinylated biomolecules (e.g. RNA or DNA) in histochemical, flow cytometry, blotting and microplate assays. The detection of Biotin relies on its high affinity (KD= 10-15 M) to Streptavidin that is labeled with the photostable, hydrophilic AF488 dye (also known as Alexa Fluor® 488). AF488 can be detected with the standard FITC filter set.
Degree of Labeling (molar dye/protein ratio): 2 - 4
Preparation of 1 mg/ml working solution:
Add 1 ml ddH2O, vortex and spin-down briefly.
 Diamandis et al. (1991) The biotin-(strept)avidin system: principles and applications in biotechnology. Clin. Chem. 37:625.