For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
protect from light
Shelf Life: 12 months
Molecular Weight: 2713 Da confirmed by ESI-MS, peptide provides 9 positive charges in side chains for complex formation. Up to 10 trifluoroacetate residues may be present resulting in an apparent MW of 3.3 kDa.
Purity: > 99 % (HPLC)
sC18 is a C-terminal fragment (aa 106-121) of the cationic amphipathic peptide CAP18. It facilitates internalization of cargo into living cells with high transduction rates and efficiencies. It shows no cytotoxic effects on a number of cell lines (including HeLa, MCF-7, HEK293, HT-29, FaDu, BJAB) up to a concentration of 100 μM. The transport of cargo requires in most cases formation of a covalent conjugate or fusion, however in some cases sC18 is able to form non-covalent complexes with the cargo as well. Addition of a 10 to 20 fold excess of free sC18 may increase rate and efficiency of cargo internalization.1-5
Cys-functionalized sC18 allows covalent coupling of thiol reactive compounds e.g.
Cystein (Cys) - functionalized peptide.
Dissolve 0.25 mg in 0.75 ml sterile and oxygen-free water according to the general manual. Use the solution immediately or aliquot and store at -20 °C. Avoid freeze / thaw cycles. 1 μl of stock solution contains 0.33 μg peptide according to 0.1 nmol.
Perform calculation, complex formation and cargo transduction according to detailed protocols given in the general manual.
Hoyer et al. (2012) Dimerization of a cell-penetrating peptide leads to enhanced cellular uptake and drug delivery. Beilstein J. Org. Chem. 8:1788.
 Richter et al. (2012) (18)F-Labeled phosphopeptide-cell-penetrating peptide dimers with enhanced cell uptake properties in human cancer cells. Nucl. Med. Biol. 39:1202.
 Splith et al. (2012) Specific targeting of hypoxic tumor tissue with nitroimidazole-peptide conjugates. ChemMedChem 7:57.
 Splith et al. (2010) Protease-activatable organometal-Peptide bioconjugates with enhanced cytotoxicity on cancer cells. Bioconjug. Chem. 21:1288.
 Reissmann (2014) Cell penetration: scope and limitations by the application of cell-penetrating peptides. J. Pept. Sci. 20:760.
 Tansi et al. (2015) Internalization of near-infrared fluorescently labeled activatable cell-penetrating peptide and of proteins into human fibrosarcoma cell line HT-1080.J. Cell. Biochem. 116:1222.
Ryan et al. (2011) Tunable reagents for multi-functional bioconjugation: reversible or permanent chemical modification of proteins and peptides by control of maleimide hydrolysis. Chem. Commun. 47:5452.
 Badescu et al. (2014) A new reagent for stable thiol-specific conjuagtion. Bioconjugate Chem. 25:460.
 Smeenk et al. (2012) Synthesis of water-soluble scaffolds for peptide cyclization, labeling and ligation. Organic Lett. 14(5):1194.
 Dewkar et al. (2012) Synthesis of novel peptide linkers: simultaneous cyclization and labeling. Organic Lett. 11(20):4708.