For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
protect from light
Shelf Life: 12 months after date of delivery
Molecular Weight: 3392 Da confirmed by ESI-MS, peptide provides 10 positive charges. Up to 10 trifluoroacetate residues may be present resulting in an apparent MW of 4.55 kDa.
Purity: > 99 % (HPLC)
Activity: 1 μl of stock solution forms a non-covalent complex with 0.1 μmol avidin or streptavidin containing cargo. Try to avoid formation of large size aggregates: An equimolar ratio of biotin and avidin/streptavidin groups is recommended as starting point. Please keep in mind: Avidin/Streptavidin contain 4 biotin binding sites.
hCT(18-32)-k7 is a cell-penetrating peptide derived from human calcitonin. It contains a nuclear localisation sequence and facilitates internalization of cargo into living cells with high transduction rates and efficiencies. hCT(18-32)-k7 shows only small cytotoxic effects on a number of cell lines (including HeLa, Jurkat, HEK293, MCF-7, SK-N-MC, COS-7) and can be used for internalization of DNA, pDNA, ribonucleic acids and proteins. For cell survival the critical concentration of hCT(18-32)-k7 in serum-free transduction medium is in the range of 20 μg/ml at which cell viability and cell membrane integrity are only marginally reduced (approx. 10-30%).
Biotin-labeled hCT(18-32)-k7 can be used for complex formation with Avidin/Streptavidin-containing cargo and subsequent complex internalization.
Biotin - labeled peptide.
Dissolve 0.5 mg in 1.1 ml sterile and oxygen-free water according to the general manual. Use the solution immediately or aliquot and store at -20 °C. Avoid freeze / thaw cycles. 1 μl of stock solution contains 0.44 μg peptide according to 0.1 nmol.
Perform calculation, complex formation and cargo transduction according to detailed protocols given in the general manual.
Reissmann (2014) Cell penetration: scope and limitations by the application of cell-penetrating peptides. J. Pept. Sci. 20:760.
Leßig et al. (2013) Efficient inhibition of human leukocytic elastase by means of α1antitrypsin/peptide complexes. Cytometry A. 83:461.
Walther et al. (2009) Specific labeling with potent radiolabels alters the uptake of cell-penetrating peptides. Biopolymers 92:445.
Neundorf et al. (2008) Cymantrene conjugation modulates the intracellular distribution and induces high cytotoxicity of a cell-penetrating peptide. Chem. Commun. (Camb) 21:5604.
Rennert et al. (2008) Generation of carrier peptides for the delivery of nucleic acid drugs in primary cells. ChemMedChem 3:241.
Rennert et al. (2008) Synthesis and application of peptides as drug carriers. Methods Mol. Biol. 535:389.
Walther et al. (2008) Quantum dot-carrier peptide conjugates suitable for imaging and delivery applications. Bioconjug. Chem. 2008 19:2346.