For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
protect from light
Shelf Life: 12 months after date of delivery
Molecular Weight: 2296 Da confirmed by ESI-MS, peptide provides 9 positive charges in side chains. Up to 9 trifluoroacetate residues may be present resulting in an apparent MW of 3.4 kDa.
Purity: > 99 % (HPLC)
Activity: 1 μl of stock solution forms a non-covalent complex with 0.1 μmol avidin or streptavidin containing cargo. Try to avoid formation of large size aggregates: An equimolar ratio of biotin and avidin/streptavidin groups is recommended as starting point. Please keep in mind: Avidin/Streptavidin contain 4 biotin binding sites.
sC18 is a C-terminal fragment (aa 106-121) of the cationic amphipathic peptide CAP18. It facilitates internalization of cargo into living cells with high transduction rates and efficiencies. It shows no cytotoxic effects on a number of cell lines (including HeLa, MCF-7, HEK293, HT-29, FaDu, BJAB) up to a concentration of 100 μM. Addition of a 10 to 20-fold excess of free sC18 increases rate and efficiency of cargo internalization.
Biotin-labeled sC18 can be used for complex formation with Avidin/Streptavidin-containing cargo and subsequent complex internalization.
Biotin - labeled peptide.
Dissolve 0.5 mg in 1.5 ml sterile and oxygen-free water according to the general manual. Use the solution immediately or aliquot and store at -20 °C. Avoid freeze / thaw cycles. 1 μl of stock solution contains 0.32 μg peptide according to 0.1 nmol.
Perform calculation, complex formation and cargo transduction according to detailed protocols given in the general manual.
Reissmann (2014) Cell penetration: scope and limitations by the application of cell-penetrating peptides. J. Pept. Sci. 20:760.
Hoyer et al. (2012) Dimerization of a cell-penetrating peptide leads to enhanced cellular uptake and drug delivery. Beilstein J. Org. Chem. 8:1788.
Richter et al. (2012) (18)F-Labeled phosphopeptide-cell-penetrating peptide dimers with enhanced cell uptake properties in human cancer cells. Nucl. Med. Biol. 39:1202.
Splith et al. (2012) Specific targeting of hypoxic tumor tissue with nitroimidazole-peptide conjugates. ChemMedChem 7:57.
Splith et al. (2010) Protease-activatable organometal-Peptide bioconjugates with enhanced cytotoxicity on cancer cells. Bioconjug. Chem. 21:1288.