Master mix of heat-activatable DNA polymerase for high specificity
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Concentration: 5x conc.
Availability Restriction: Exclusively distributed in Japan by Greiner BioOne
Hot Start Master contains all reagents required for PCR (except template and primer) in a premixed 5x concentrated ready-to-use solution.
The master mix provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room-temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during PCR setup.
Hot Start Master requires no prolonged heating or denaturing step. The polymerase inhibiting ligand is quickly released at the increased temperature of thermal cycling.
5x Hot Start Master (red cap)
master mix of heat-activatable DNA polymerase, dATP, dCTP, dGTP, dTTP, KCl, MgCl2 and stabilizers.
PCR grade water (white cap)
Recommended 50 μl PCR assay:
|10 μl||5x Taq Master Mix||red cap|
|0.2 - 1 μM||each Primer||-|
|2 - 50 ng||Template DNA||-|
|Fill up to 50 μl||PCR grade Water||white cap|
|94 °C||2 min||1x|
|Denaturation||94 °C||30 sec||30x|
|Annealing1)||45 - 68 °C||30 sec||30x|
|Elongation2)||72 °C||30 sec - 4 min||30x|
|72 °C||2 min||1x|
For optimal specificity and amplification an individual optimization of the recommended parameters may be necessary for each new template DNA and/or primer pair.