Master mix of thermostable DNA polymerase
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Concentration: 5x conc.
Availability Restriction: Exclusively distributed in Japan by Greiner BioOne
Taq Master contains all reagents required for PCR (except template and primer) in a premixed 5x concentrated ready-to-use solution.
The master mix is recommended for use in routine PCR reactions. It is optimized for high specificity and guarantees minimal by-product formation. The mix is particularly suitable for plate based PCR and automated pipetting where a detergent free buffer system is required.
The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction in the presence of magnesium. It also possesses a 5'→3' polymerization-dependent exonuclease replacement activity but lacks a 3'→5' exonuclease activity.
5x Taq Master (red cap)
master mix of thermostable DNA polymerase, dATP, dCTP, dGTP, dTTP, KCl, MgCl2 and stabilizers.
PCR grade water (white cap)
Recommended 50 μl PCR assay:
|10 μl||5x Taq Master Mix||red cap|
|0.2 - 1 μM||each Primer||-|
|2 - 50 ng||Template DNA||-|
|Fill up to 50 μl||PCR grade Water||white cap|
|94 °C||2 min||1x|
|Denaturation||94 °C||30 sec||30x|
|Annealing1)||45 - 68 °C||30 sec||30x|
|Elongation2)||72 °C||30 sec - 4 min||30x|
|72 °C||2 min||1x|
For optimal specificity and amplification an individual optimization of the recommended parameters may be necessary for each new template DNA and/or primer pair.