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Taq Master / high yield

Master mix of thermostable DNA polymerase

Product Cat. No. Amount Price (EUR) Buy / Note
Taq Master / high yield PCR-101S 1 ml (5x conc.) 40,00 Add to Basket/Quote Add to Notepad
Taq Master / high yield PCR-101L 5 x 1 ml (5x conc.) 160,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Form: liquid

Concentration: 5x conc.

Description:
Taq Master / high yield contains all reagents required for PCR (except template and primer) in a premixed 5x concentrated ready-to-use solution.
The master mix is recommended for use in routine PCR reactions. It is optimized for high efficiency and gives superior amplification results in a broad range of reaction conditions with most primer-template pairs. Note that the mix is based on a detergent containing buffer system not recommended for plate based PCR and automated pipetting.
The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction in the presence of magnesium. It also possesses a 5'→3' polymerization-dependent exonuclease replacement activity but lacks a 3'→5' exonuclease activity.

Kit contents:
5x Taq Master / high yield (red cap)
master mix of thermostable DNA polymerase, dATP, dCTP, dGTP, dTTP, (NH4)2SO4, MgCl2, Tween-20, Nonidet P-40 and stabilizers.

PCR grade water (white cap)

Recommended 50 μl PCR assay:

10 μl5x Taq Master Mixred cap
0.2 - 1 μMeach Primer-
2 - 50 ngTemplate DNA-
Fill up to 50 μlPCR grade Waterwhite cap

Recommended cycling conditions:
Initial
denaturation
94 °C2 min1x
Denaturation94 °C30 sec30x
Annealing1)45 - 68 °C30 sec30x
Elongation2)72 °C30 sec - 4 min30x
Final
elongation
72 °C2 min1x
1)The annealing temperature depends on the melting temperature of the primers used.
2)The elongation time depends on the length of the fragments to be amplified. A time of 1 min/kbp is recommended.

For optimal specificity and amplification an individual optimization of the recommended parameters may be necessary for each new template DNA and/or primer pair.

Availability Restriction: Exclusively distributed in Japan by Greiner BioOne