dCTP - Solution
For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).
Description:
dCTP, PCR-grade is supplied as ultrapure aqueous solution (pH 8.5) and suitable for all molecular biology applications including PCR/qPCR, reverse transcription, DNA labeling and DNA sequencing.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Shelf Life: 12 months
Molecular Formula: C9H16N3O13P3 (free acid)
Molecular Weight: 467.15 g/mol (free acid)
CAS#: 102783-51-7
Purity: ≥ 99 % (HPLC)
Form: clear aqueous solution
Concentration: 100 mM - 110 mM
pH: 8.5 ±0.2 (22 °C)
Spectroscopic Properties: λmax 271 nm, ε 8.9 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Selected References:
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.
| Cat. No. | Amount | Price |
|---|---|---|
| NU-1002L | 1 ml (100 mM) | 96,60 € |
| NU-1002-10ML | 10 ml (100 mM) | 675,90 € |
| NU-1002-100ML | 100 ml (100 mM) | Ask for Quotation |
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Scientific citations for Jena Bioscience nu-1002
13 scientific citations and 5 citation images found by Bioz.
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Modified DNA substrate selectivity by GmrSD-family Type IV restriction enzyme BrxU.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2025-09-04 · PMID:40904119
.. BrxU assays Synthetically modified substrates were produced by PCR using pUC19 as a template.. Amplification was carried out replacing
deoxycytidine triphosphate (dCTP) with either 5hmdC or 5mdC (Jena Biosciences ).. The 2.7 kb amplicon was purified using a Monarch PCR Cleanup Kit (NEB).The 2.7 .. -
A mobile genetic element-derived primase-polymerase harbors multiple activities implicated in DNA replication and repair
Nucleic Acids Research, 2025-01-27 · DOI:10.1093/nar/gkae1318
Gel-based primase analysis were conducted in a 10 μl volume, containing 20 mM Tris–HCl, pH 6.5, 10 mM MnCl 2 , 0.1 mg/ml BSA, 1 μM single-stranded DNA (ssDNA) (DNA-KZ53) or single-stranded RNA (ssRNA) (RNA-KZ53) or 50 ng M13 ssDNA as template, 1 μM AgaPP protein or its variants, 100 μM unlabeled NTPs, 2.5 μM unlabeled dNTPs and NTPs with either FAM-labeled UTP or FAM-labeled
dCTP (Jena Bioscience , Dortmund, Germany). -
Notch1 sets the molecular clock for T cell development
Europe PMC, 2024-09-24 · DOI:
.. of DpnII (NEB) for 4h at 37°C, following heat inactivation for 20min at 62°C..
Fragment overhangs were filled, and DNA ends marked with biotin using dCTP (Jena Bioscience NU1002L), dGTP (Jena Bioscience NU-1003L), dTTP, bio-dATP (Jena Bioscience NU-835-Bio14-S), and 50U DNA Pol.. I, Large Klenow Fragment (Jena Bioscience EN-148S) for 1h at 37°C. fragments were ligated ..
