dNTP Mix - 25 mM Solution

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Description:
dNTP Mix is an equimolar mixture of ultrapure dATP, dCTP, dGTP, and dTTP supplied as clear aqueous solution (pH 8.5).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C

Shelf Life: 12 months

Molecular Formula:
dATP: C₁₀H₁₆N₅O₁₂P₃ (free acid)
dCTP: C₉H₁₆N₃O₁₃P₃ (free acid)
dGTP: C₁₀H₁₆N₅O₁₃P₃ (free acid)
dTTP: C₁₀H₁₇N₂O₁₄P₃ (free acid)

Molecular Weight:
dATP: 491.18 g/mol (free acid)
dCTP: 467.15 g/mol (free acid)
dGTP: 507.18 g/mol (free acid)
dTTP: 482.17 g/mol (free acid)

Purity: ≥ 99 % (HPLC)

Form: clear aqueous solution

pH: 8.5 ±0.2 (22 °C)

Applications:
For standard PCR applications a final concentration of 200 μM each dNTP is recommended.

Quality Control Specifications:
Low Copy Long Range PCR (18 kb, lambda DNA, template dilution series): PCR fragment with 50 pg of template
RT-PCR (749 bp fragment, human GAPDH gene, template dilution series): PCR fragment with 10 pg of template
Contamination with bacterial or human DNA: not detectable
DNases, RNases, Nicking Activity: not detectable
Proteases: not detectable

Selected References:
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.