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5-Propargylamino-dCTP - Solution

5-Propargylamino-2'-deoxycytidine-5'-triphosphate, Sodium salt

Cat. No. Amount Price (EUR) Buy / Note
NU-809S 10 μl (100 mM)74,60 Add to Basket/Quote Add to Notepad
NU-809L 5 x 10 μl (100 mM)336,50 Add to Basket/Quote Add to Notepad
NU-809XL 100 μl (100 mM)558,80 Add to Basket/Quote Add to Notepad
Structural formula of 5-Propargylamino-dCTP - Solution (5-Propargylamino-2'-deoxycytidine-5'-triphosphate, Sodium salt)
Structural formula of 5-Propargylamino-dCTP - Solution

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C12H19N4O13P3 (free acid)

Molecular Weight: 520.22 g/mol (free acid)

Exact Mass: 520.02 g/mol (free acid)

CAS#: 115899-39-3

Purity: ≥ 93 % (HPLC)

Form: solution in water

Color: slightly yellow

Concentration: 100 mM - 110 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Incorporation into DNA by

  • PCR with Taq polymerasein-house data

Propargylamino-dCTP is recommended for two-step labeling of DNA e.g. by PCR. It is enzymatically incorporated into DNA as substitute for its natural counterpart dCTP. The resulting Amine-functionalized DNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice

  • to introduce a Biotin group (via NHS Ester of Biotin) for subsequent purification tasks
  • to introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging

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