5-Hydroxymethyl-2'-deoxycytidine-5'-triphosphate, Sodium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C10H18N3O14P3 (free acid)
Molecular Weight: 497.18 g/mol (free acid)
Purity: ≥ 95 % (HPLC), < 0.5% dCTP (HPLC)
Form: clear aqueous solution
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 275 nm, ε 7.8 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Incorporation into DNA by
- PCR with Taq polymerase in-house data, [1-6]
5-Hydroxymethylated DNA probes can be used as sequencing control [1-5] or for pull-down of 5-hmC binding proteins from cellular lysate.
 Booth et al. (2014) Quantitative sequencing of 5-formylcytosine in DNA at single-base resolution. Nat. Chem. 6 (5):435.
 Booth et al. (2013) Oxidative bisulfite sequencing of 5-methylcytosine and 5-hydroxymethylcytosine. Nat. Protoc. 8 (10):1841.
 Yu et al. (2012) Tet-assisted bisulfite sequencing of 5-hydroxymethylcytosine. Nat. Protoc. 7 (12):2159.
 Szwagierczak et al. (2011) Characterization of PvuRts1I endonuclease as a tool to investigate genomic 5-hydroxymethylcytosine. Nucl. Acids Res. 39(12):5149.
 Szwagierczak et al. (2010) Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA. Nucl. Acids Res. 38(19):e181.
 Lafaye et al. (2014) DNA binding of the p21 repressor ZBTB2 is inhibited by cytosine hydroxymethylation. Biochem. Biophys. Res. Commun. 446:341.