5-Propargylamino-2'-deoxycytidine-5'-triphosphate, Sodium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C12H19N4O13P3 (free acid)
Molecular Weight: 520.22 g/mol (free acid)
Purity: ≥ 93 % (HPLC)
Form: slightly yellowish aqueous solution
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Incorporation into DNA by
Propargylamino-dCTP is recommended for two-step labeling of DNA e.g. by PCR. It is enzymatically incorporated into DNA as substitute for its natural counterpart dCTP. The resulting Amine-functionalized DNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice