8-Oxo-dGMP
For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C10H14N5O8P (free acid)
Molecular Weight: 363.22 g/mol (free acid)
Exact Mass: 363.06 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 245 nm, ε 12.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Crystal structure in complex with human MTH1[1]
Hydrolysis by MutT[2]
Marker of oxidative stress[3]
Parameters of pyrophposphohydrotase reaction of MutT[4]
Solution structure of complex with MutT[5]
Specific Ligands:
Tight binding to MutT[6]
Selected References:
[1] Svensson et al. (2011) Crystal structure of human MTH1 and 8-oxo-dGMP product complex. FEBS Letters 585:2617.
[2] Nakamura et al. (2010) Structural and dynamic features of the MutT protein in the recognition of nucleotides with the mutagenic 8-oxoguanine base. J. Biological Chem. 285:444.
[3] Sangsuwan et al. (2008) The nucleotide pool, a target for low-dose y-ray induced oxidative stress. Radiation Research 170:776.
[4] Xia et al. (2005) Transient state kinetic studies of the MutT-catalyzed nucleoside triphosphate pyrophosphohydrolase reaction. Biochemistry 44:15334.
[5] Massiah et al. (2004) Solution structure, mutagenesis, and NH-exchange studies of the MutT enzyme Mg2+-8-oxo-GMP complex. J. Molecular Structure 700 (1-3):247.
[6] Saraswat et al. (2004) Mutational, NMR, and NH-exchange studies of the tight and selective binding of 8-oxo-GMP by the MutT pyrophosphohydrolase. Biochemistry 43:3404.
Kaminsky et al. (2007) The c-Myc Target Gene Rcl (C6orf108) Encodes a Noval Enzyme, Deoxynucleoside 5'-monophosphate N-Glycosidase. J. Biol. Chem. 282 (11): 8150.
| Cat. No. | Amount | Price |
|---|---|---|
| NU-1153S | 50 μl (10 mM) | 157,60 € |
| NU-1153L | 5 x 50 μl (10 mM) | 461,30 € |
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Scientific citations for Jena Bioscience nu-1153
2 scientific citations and 0 citation images found by Bioz.
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An integrated approach to evaluate acetamiprid-induced oxidative damage to tRNA in human cells based on oxidized nucleotide and tRNA profiling.
Environment international, 2023-08-21 · PMID:37343327
8-oxo-GTP (10 mM), 8-oxo-dGTP (10 mM), 8-oxo-2,
7-dihydro-2′deoxyguanosine diphosphate (8-oxo-dGDP, 10 mM), 8-oxo-2, 7-dihydro-2′-deoxyguanosine monophosphate (8-oxo-dGMP, 10 mM), 8-oxodGuo (25 mg), 8-oxo-adenosine triphosphate (8-oxo-ATP, 100 mM) and 8-oxo-deoxyadenosine triphosphate (8-oxo-dATP, 100 mM) were purchased fromJena BioScience (Jena, Germany). -
Rice Nudix Hydrolase OsNUDX2 Sanitizes Oxidized Nucleotides
Antioxidants, 2022-09-13 · PMID:36139879
.. at 25 °C, and measured for absorbance at 620 nm.. 8-oxo-dGTP, 8-oxo-dGDP,
8-oxo-dGMP (Jena Bioscience , Thuringia, Germany), and the product after reaction toward 8-oxo-dGTP were analyzed using HPLC with a Cosmosil C 18 column (4.6 × 250 mm; Nacalai Tesque, Kyoto, Japan) equilibrated with 100 mM phosphate buffer (pH 6.0) and 5% ( v/v ) methanol, at a flow rate of 1.0 mL/min.. The substrate and the reaction product were detected by absorption ..
