Guanosine-5'-[( β,γ )-imido]triphosphate, Trisodium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 6 months after date of delivery
Molecular Formula: C10H17N6O13P3 (free acid)
Molecular Weight: 522.19 g/mol (free acid)
Purity: ≥ 90 % (HPLC)
Spectroscopic Properties: λmax 252 nm, ε 13.7 L mmol-1 cm-1 (Tris-HCl pH 7.5)
X-ray elongation factor EF-G
X-ray with Rab27
Conformational switch of IF(α,β,y)
Dynamic of ribosomes
Intitiation factors IF2, IF(α,β,y) and elongation factor EF-G[5, 6]
Please note: For reasons of stability, please make sure that the pH value of a solution of this product never drops below 7.0. This can be achieved by dissolving the nucleotide in a buffer of your choice (50 - 100 mM, pH 7 - 10). Dissolve and adjust concentration photometrically.
When stored at -20 °C, product may hydrolyze, thereby forming GppNH2 at a rate of up to 1 % per month!
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 Chavas et al. (2009) Structural insights into Rab27 recruitment by ist effectors. Nippon Kessho Gakkaishi 51:334.
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 Burakovsky et.al. (2007) The interaction with Escherichia coli 23S rRNA helices 89 and 91 contributes to the IF2 activity but is insignificant for the functioning of the elongation factors. Mol. Biol. 41:939.
 Spiegel et al. (2007) Elongation factor G stabilizes the hybrid-state conformation of 70S ribosome. RNA 13:1473.
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