pCp-Desthiobiotin
For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).
Description:
pCp-Desthiobiotin is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single Desthiobiotin molecule to the 3'-OH group of the RNA/miRNA template. The resulting Desthiobiotin-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA). Detection of the Desthiobiotin moiety can be performed using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Alternatively, Desthiobiotin-labeled RNA/miRNA probes can also be purified with Streptavidin-conjugated agarose/magnetic beads.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C25H44N6O13P2 (free acid)
Molecular Weight: 698.60 g/mol (free acid)
Exact Mass: 698.24 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in 10 mM Tris-HCl
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 271 nm, ε 8.9 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Incorporation into RNA/miRNA by
- 3'-End Labeling with T4 RNA Ligase
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