pCp-Biotin

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Description:
pCp-Biotin is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single Biotin molecule to the 3'-OH group of the RNA/miRNA template. The resulting Biotin-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA). Detection of the Biotin moiety can be performed using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Alternatively, Biotin-labeled RNA/miRNA probes can also be purified with Streptavidin-conjugated agarose/magnetic beads.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₂₅H₄₂N₆O₁₃P₂S₁ (free acid)

Molecular Weight: 728.65 g/mol (free acid)

Exact Mass: 728.20 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solution in 10 mM Tris-HCl

Color: colorless to slightly yellow

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 271 nm, ε 8.9 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into RNA/miRNA by

• 3'-End Labeling with T4 RNA Ligase

Related products:

• pCp-Desthiobiotin, #NU-1706-Desthiobio
• pCp-Cy3, #NU-1706-CY3
• pCp-Cy5, #NU-1706-CY5
• pCp-Amine, #NU-1706
• pCp-Azide, #NU-1708
• pCp-Alkyne, #NU-1709

Selected References:
Kashida et al. (2018) Nanoparticle-based local translation reveals mRNA as translation-coupled scaffold with anchoring function. doi:10.1101/483727.
Booy et al. (2016) RNA Helicase Associated with AU-rich Element (RHAU/DHX36) Interacts with the 3′-Tail of the Long Non-coding RNA BC200 (BCYRN1). J. Biol. Chem. 291 (10):5355.