m⁷GDP - Solution

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₁H₁₇N₅O₁₁P₂ (free acid)

Molecular Weight: 457.23 g/mol (free acid)

Exact Mass: 457.04 g/mol (free acid)

CAS#: 117723-13-4 (free acid), 104809-16-7 (sodium salt)

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 258/280 nm, ε 9.8/8.0 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
Inhibition of cap-dependent translation^[1]
Inhibition of mitotic division^[2]
Inhibition of decapping^[3]

BIOZ Product Citations:

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Selected References:
[1] Allam et al. (2010) Initiation factor eIF2- independent mode of c-src mRNA translation occurs via an internal ribosome entry site. J. Biol. Chem. 285:5713.
[2] Oulhen et al. (2007) After fertilization of sea urchin eggs, eIF4G is post-translationally modified and associated with the cap-binding protein eIF4E. J. Cell Science 120:425.
[3] Parrish et al. (2007) Vaccinia virus D10 protein has mRNA decapping activity, providing a mechanism for control of host and viral gene expression. Proc. Natl. Acad. Sci. (USA) 104:2139.
Shen et al. (2001) Structural and thermodynamic behavior of eukaryotic initiation factor 4E in supramolecular formation with 4E-binding protein 1 and mRNA cap analogue, studied by spectroscopic methods. Chem Pharm Bull 49 (10):1299.
Carberry et al. (1989) A spectroscopic study of the binding of m7GTP and m7GpppG to human protein synthesis initiation factor 4E. Biochemistry 28 (20):8078.
Beemon et al. (1977) In vitro translation yields a possible Rous sarcoma virus src gene product. Proc Natl Acad Sci U S A. 74 (8):3302.