5-Methyl-dCMP

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₀H₁₆N₃O₇P (free acid)

Molecular Weight: 321.23 g/mol (free acid)

Exact Mass: 321.07 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 277 nm, ε 9.0 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

BIOZ Product Citations:

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Selected References:
Kamiya et al. (2004) Important amino acids in the phosphohydrolase module of Escherichia coli Orf135. Biochem Biophys Res Commun. 323 (3):1063.
Holliday et al. (2002) DNA methylation and epigenetic inheritance. Methods. 27 (2):179.
Kaito et al. (2001) Activation of the maternally preset program of apoptosis by microinjection of 5-aza-2'-deoxycytidine and 5-methyl-2'-deoxycytidine-5'-triphosphate in Xenopus laevis embryos. Dev Growth Differ. 43 (4):383.
Wong et al. (1997) A novel method for producing partial restriction digestion of DNA fragments by PCR with 5-methyl-CTP. Nucleic Acids Res. 25 (20):4169.
Chen et al. (1993) Direct induction of DNA hypermethylation in sea urchin embryos by microinjection of 5-methyl dCTP stimulates early histone gene expression and leads to developmental arrest. Dev Biol. 155 (1):75.
Gehrke et al. (1984) Quantitative reversed-phase high-performance liquid chromatography of major and modified nucleosides in DNA. J. Chromatogr. 301:199.
Eick et al. (1983) Quantitative determination of 5-methylcytosine in DNA by reverse-phase high-performance liquid chromatography. Anal. Biochem. 135:165.