5-Hydroxymethyl-dCTP

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Description:
5-Hydroxymethylated DNA probes can be used as sequencing control ^[1-5] or for pull-down of 5-hmC binding proteins from cellular lysate^[6].

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₀H₁₈N₃O₁₄P₃ (free acid)

Molecular Weight: 497.18 g/mol (free acid)

Exact Mass: 497.00 g/mol (free acid)

CAS#: 7226-77-9

Purity: ≥ 95 % (HPLC), < 0.5% dCTP (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 100 mM - 110 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 275 nm, ε 7.8 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into DNA by
- PCR with Taq polymerase ^{in-house data, [1-6]}

Selected References:
[1] Booth et al. (2014) Quantitative sequencing of 5-formylcytosine in DNA at single-base resolution. Nat. Chem. 6 (5):435.
[2] Booth et al. (2013) Oxidative bisulfite sequencing of 5-methylcytosine and 5-hydroxymethylcytosine. Nat. Protoc. 8 (10):1841.
[3] Yu et al. (2012) Tet-assisted bisulfite sequencing of 5-hydroxymethylcytosine. Nat. Protoc. 7 (12):2159.
[4] Szwagierczak et al. (2011) Characterization of PvuRts1I endonuclease as a tool to investigate genomic 5-hydroxymethylcytosine. Nucl. Acids Res. 39(12):5149.
[5] Szwagierczak et al. (2010) Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA. Nucl. Acids Res. 38(19):e181.
[6] Lafaye et al. (2014) DNA binding of the p21 repressor ZBTB2 is inhibited by cytosine hydroxymethylation. Biochem. Biophys. Res. Commun. 446:341.