3'-O-Azidomethyl-dCTP - Solution

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₀H₁₇N₆O₁₃P₃ (free acid)

Molecular Weight: 522.20 g/mol (free acid)

Exact Mass: 522.01 g/mol (free acid)

Purity: ≥ 98 % (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 10 mM - 11 mM

pH: 8.2 ±0.2

Spectroscopic Properties: λ_max 271 nm, ε 8.9 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Selected References:
Palla et al. (2014) DNA sequencing by synthesis using 3'-O-azidomethyl nucleotide reversible terminators and surface-enhanced Raman spectroscopic detection. RSC Adv. 4 (90):49342.
Zavgorodny et al. (1991) Alkylthioalkylation of Nucleoside Hydroxyl Functions and Its Synthetic Applications: A New Versatile Method in Nucleoside Chemistry. Tetrahedron Letters 32 (51):7593.