Aminoallyl-dUTP-XX-Cy3

Excitation and Emission spectrum of Cy3

For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).

Description:
Aminoallyl-dUTP-XX-Cy3 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Higher incorporation and thus labeling rates can be achieved compared to Aminoally-dUTP-Cy3 (NU-803-Cy3).

Recommended Aminoallyl-dUTP-XX-Cy3/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-XX-Cy3/ 50% dTTP

Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₅₅H₇₈N₇O₂₃P₃S₂ (free acid)

Molecular Weight: 1362.29 g/mol (free acid)

Exact Mass: 1361.38 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solution in 10 mM Tris-HCl

Color: pink

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_exc 550 nm, λ_em 570 nm, ε 150.0 L mmol^-1 cm^-1 (Tris-HCl pH 7.5), CF₂₆₀ 0.08

Applications:
Incorporation into DNA/cDNA by
- PCR with Taq polymerase ^{in-house data}
- Nick Translation with DNAse I/ DNA Polymerase I ^{in-house data}

Related products:

• HighFidelity Cy3 PCR Labeling Kit, #APP-101-Cy3
• HighFidelity YELLOW PCR Labeling Testkit, #APP-101-YELLOW
• Cy3 NT Labeling Kit, #PP-305-Cy3