also known as BODIPY® FL GTPγS
Guanosine-5'-(γ-thio)-triphosphate, labeled with BODIPY® FL, Sodium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C24H30BF2N8O14P3S (free acid)
Molecular Weight: 828.33 g/mol (free acid)
Exact Mass: 828.09 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: orange clear aqueous solution
Concentration: 5.0 mM - 5.5 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λexc 503 nm, λem 512 nm, ε 80.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Mancini et al. (2007) Exploring the Technology Landscape of 7TMR Drug Signaling Profiling. Curr. Top. Med. Chem. 15 (24):2528
Vadakkadathmeethal et al. (2007) Fluorescence-based adenylyl cyclase assay adaptable to high throughput screening. Comb. Chem. High Throughput Screen. 10 (4):289.
Korlach et al. (2004) Spontaneous nucleotide exchange in low molecular weight GTPases by fluorescently labeled gamma-phosphate-linked GTP analogs. Proc. Natl. Acad. Sci. USA 101 (9):2008.
Whelan et al. (2004) Affinity assays using fluorescence anisotropy with capillary electrophoresis separation. Anal. Chem. 76 (24):7380.
Gill et al. (2003) Low-affinity interactions of BODIPY-FL-GTPgammaS and BODIPY-FL-GppNHp with G(i)- and G(s)-proteins. Naunyn Schmiedebergs Arch. Pharmacol. 368 (3):210.
McEwen et al. (2001) Fluorescent BODIPY-GTP analogs: real-time measurement of nucleotide binding to G proteins. Anal. Biochem. 291 (1):109.