EDA-GTP-ATTO-680
excitation and emission spectrum of ATTO 680
For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C40H51N10O20P3S (free acid)
Molecular Weight: 1116.88 g/mol (free acid)
Exact Mass: 1116.22 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: blue
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λexc 681 nm, λem 698 nm, ε 125.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
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Scientific citations for Jena Bioscience nu-820-680
2 scientific citations and 0 citation images found by Bioz.
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Oxidative stress influences positive strand RNA virus genome synthesis and capping
Virology, 2014-12-13 · PMID:25514423
.. as previously described ( ).Protein guanylation assays were performed as previously described ( ).. Briefly, the 3 μM enzyme was incubated with
1 μM GTP-ATTO 680 (Jena Bioscience , Germany) in 5 mM Tris-base (pH 7.5), 500 nM MgCl 2 and 0.1% NP-40 for 120 min at 37 °C.. We have observed that VEEV nsP1 (New World Alphavirus) does not require S -adenosylmethionine .. -
Development of novel alphavirus expression and detection systems and characterization of oxidation's effect on viral replication
, 2014-01-01 · DOI:
.. guanylation assay Protein guanylation assays were performed as previously described (Geiss et al. 2011).. Briefly, the enzyme (3 (M) was incubated with
1 uM GTP-ATTO 680 (Jena Bioscience , Germany) in 5 mM Tris-base (pH 7.5), 500 nM MgClz and 0.1% NP-40 for 120 minutes at 37°C.. The reactions were resolved on 12.5% polyacrylamide gels, and the extent of protein guanylation ..
