5-Methylcytidine-5'-triphosphate, Sodium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C10H18N3O14P3 (free acid)
Molecular Weight: 497.18 g/mol (free acid)
CAS#: 327174-86-7 (acid)
Purity: ≥ 95 % (HPLC)
Form: clear aqueous solution
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 277 nm, ε 9.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Functional influence of modified bases[1-5]
Incorporation by PCR
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 Adams et al. (2014) 185 CELLULAR VIABILITY AND EXPRESSION OF GREEN FLUORESCENT PROTEIN IN BOVINE FETAL FIBROBLASTS FOLLOWING TRANSFECTION OF SYNTHETIC mRNA INCLUDING MODIFIED BASES. Reprod. Fertil. Dev. 26:207.
 Tchigvintsev et al. (2013) Biochemical and Structural Studies of Conserved Maf Proteins Revealed Nucleotide Pyrophosphatases with a Preference for Modified Nucleotides. Chem. Biol. Oxford U.K. 20:1386.
 Wong et al. (1997) A novel method for producing partial restriction digestion of DNA fragments by PCR with 5-methyl-CTP. Nucleic Acids Res. 25:4169.
 Kormann et al. (2011) Expression of therapeutic proteins after delivery of chemically modified mRNA in mice. Nature Biotechnology 29:154.
 Warren et al. (2010) Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell 7 (5):618.