pCp-Cy3

Excitation and Emission spectrum of Cy3

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Description:
pCp-Cy3 is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single dye molecule to the 3'-OH group of the RNA/miRNA template. The resulting Dye-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA).

Please note: Protect the Dye-labeled pCp from exposure to light and carry out experimental procedures under low light conditions.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₄₆H₆₄N₆O₁₈P₂S₂ (free acid)

Molecular Weight: 1115.11 g/mol (free acid)

Exact Mass: 1114.32 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solution in 10 mM Tris-HCl

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_exc 550 nm, λ_em 570 nm, ε 150.0 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into RNA/miRNA by

• 3'-End Labeling with T4 RNA Ligase^[1]

Related products:

• pCp-BIO, #NU-1706-BIO
• pCp-Desthiobiotin, #NU-1706-Desthiobio
• pCp-Cy5, #NU-1706-CY5
• pCp-Amine, #NU-1706
• pCp-Azide, #NU-1708
• pCp-Alkyne, #NU-1709

Selected References:
[1] Wanget al. (2007) Direct and sensitive miRNA profiling from low-input total RNA. RNA 13 (1):151.