In vitro synthesis of RNA is catalyzed by bacteriophage (SP6, T7 or T3) RNA polymerases using linear DNA as a template. Ribonucleoside triphosphates (NTPs) are the building blocks of these in vitro transcription reactions.
Since amplification sensitivity, product yield and reproducibility are strongly affected by contaminating impurities, the quality of NTPs is of considerably importance.
Each lot of Jena Bioscience's ultrapure (> 99 %) NTPs is confirmed free of DNase, RNase, Protease and nicking activity. Furthermore, all NTPs are functionally tested by T7 RNA Polymerase-mediated in vitro transcription.
If you require large amounts (> 100 ml, > 1 l), additional bulk discounts are available. Please contact us at nucleotides@jenabioscience.com to receive an individual quotation.