AP6A
For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C20H30N10O25P6 (free acid)
Molecular Weight: 996.35 g/mol (free acid)
Exact Mass: 995.98 g/mol (free acid)
CAS#: 56983-23-4
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 259 nm, ε 27.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Kinetic parameters with arabidopsis thaliana Nudix hydrolase[1]
Action as neurotransmitter[2]
Modeling of binding to Nudix hydrolase Ndx1[3]
Mediated release of atrial atriopeptin (ANP)[4]
Specific Ligands:
Binding properties to Nudix hydrolase[5]
Selected References:
[1] Olejnik et al. (2007) Cloning and characterization of AtNUDT13, a novel mitochondrial Arabidopsis thaliana Nudix hydrolase specific for long-chain diadenosine polyphosphates. FEBS J. 274 (18):4877.
[2] Delicado et al. (2006) Dinucleoside polyphosphates and their interaction with other nucleotide signaling pathways. Pflugers Arch. 452 (5):563.
[3] Zheng et al. (2005)Homology modeling and substrate binding study of Nudix hydrolase Ndx1 from Thermos thermophilus HB8. Biochem. Biophys. Res. Commun. 333 (3):881.
[4] Yuan et al. (2007) Diadenosine tetraphosphate stimulates atrial ANP release via A (1) receptor: involvement of K (ATP) channel and PKC. Peptides 28 (7):1397.
[5] Garza et al. (2009) Kinetic, dynamic, ligand binding properties, and structural models of a dual-substrate specific nudix hydrolase from Schizosaccharomyces pombe. Biochemistry 48 (26):6224.
Gross et al. (2006) Nucleotide-binding domains of Cystic Fibrosis Transmembrane Conductance Regulator, an ABC Transporter, Catalyze Adenylate Kinase Activity but not ATP Hydrolysis. J. Biol. Chem. 281 (7):4058.
Luo et al. (1999) Identification of diadenosine hexaphosphate in human erythrocytes. Hypertension 34:872.
Van der Giet et al. (1999) Evidence for two different P2X-receptors mediating vasoconstriction of Ap5A and Ap6A in the isolated perfused rat kidney. Br. J. Pharmacol. 127:1463.
Fontes et al. (1999) Acyl-CoA synthetase catalyzes the synthesis of diadenosine hexaphosphate (Ap6A). Biochimie 81:229.
Schluter et al. (1994) Diadenosine phosphates and the physiological control of blood pressure. Nature 367:186.
Morii et al. (1992) Adenosine (5')hexaphospho (5')adenosine stimulation of a Ca (2+)-induced Ca (2+)-release channel from skeletal muscle sarcoplasmic reticulum. Eur. J. Biochem. 205:979.
| Cat. No. | Amount | Price |
|---|---|---|
| NU-509S | 20 μl (10 mM) | 157,60 € |
| NU-509L | 5 x 20 μl (10 mM) | 461,30 € |
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Scientific citations for Jena Bioscience nu-509
3 scientific citations and 0 citation images found by Bioz.
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Lysyl-tRNA synthetase from Myxococcus xanthus catalyzes the formation of diadenosine penta- and hexaphosphates from adenosine tetraphosphate.
Archives of biochemistry and biophysics, 2017-05-09 · PMID:27392456
Abstract: Myxococcus xanthus lysyl-tRNA synthetase (LysS) produces diadenosine tetraphosphate (Ap4A) from ATP in the presence of Mn(2+); in the present study, it also generated Ap4 from ATP and triphosphate. When ATP and Ap4 were incubated with LysS and pyrophosphatase, first Ap4A, Ap5A, and ADP, and then Ap5, Ap6A, and Ap3A were generated. The results suggest that in the first step, LysS can form lysyl-AMP and lysyl-ADP intermediates from Ap4 and release triphosphate and diphosphate, respectively, whereas in the second step, it can produce Ap5 from lysyl-ADP with triphosphate, and Ap6A from lysyl-ADP with Ap4
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Cystathionine β-Synthase (CBS) Domain-containing Pyrophosphatase as a Target for Diadenosine Polyphosphates in Bacteria
Journal of Biological Chemistry, 2015-11-01 · DOI:10.1074/jbc.m115.680272
.. in terms of the dimer.All enzyme concentrations are given in terms of the dimer.. P1,Pn-diadenosine 5′-polyphosphates (ApnAs) with n = 3–5 were from Sigma;
Ap6A was fromJena Bioscience .. All ApnAs were ≥97 % pure and Ap3A was essentially free of other ApnAs, .. -
Nucleotide-binding Domains of Cystic Fibrosis Transmembrane Conductance Regulator, an ABC Transporter, Catalyze Adenylate Kinase Activity but Not ATP Hydrolysis
Journal of Biological Chemistry, 2006-02-01 · DOI:10.1074/jbc.m511113200
IC50 values for the ATP competitive inhibitor P1-(5 - adenosyl)P5-(5 -adenosyl) pentaphosphate (AP5A) (Sigma), AP5G, AP4A,
AP6A (Jena Bioscience ), were obtained by measuring the decrease in linear rates of adenylate kinase activity ( M/min) at increasing concentrations of inhibitor and fitting the data using a two parameter hyperbolic fit: y IC50/(B (X IC50)), whereB is proportional to 1/Vmax, y is the rate of inhibition ( M/min), and X is the concentration of the inhibitor.
