Reversibly 3’-O-blocked dNTPs are key components of short read sequencing technologies such as reversible termination sequencing-by-synthesis (SBS).[1-3]
[1] Palla et al. (2014) DNA sequencing by synthesis using 3′-O-azidomethyl nucleotide reversible terminators and surface-enhanced Raman spectroscopic detection. RSC Adv. 4(90):49342.
[2] Bentley et al. (2008) Accurate whole human genome sequencing using reversible terminator chemistry. Nature 456:53.
[3] Chen et al. (2013) The history and advances of reversible terminators used in new generations of sequencing technology. Genomics Proteomics Bioinformatics 11(1): 34.
