ddNTP Bundle

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Description:
ddNTP Bundle contains four separate solutions of ddATP, ddCTP, ddGTP, and ddTTP at a concentration of 10-11 mM supplied as clear aqueous solutions (pH 7.5 ±0.5).

ddATP (#NU-1015)
ddCTP (#NU-1016)
ddGTP (#NU-1017)
ddTTP (#NU-1018)

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula:
ddATP: C₁₀H₁₆N₅O₁₁P₃ (free acid)
ddCTP: C₉H₁₆N₃O₁₂P₃ (free acid)
ddGTP: C₁₀H₁₆N₅O₁₂P₃ (free acid)
ddTTP: C₁₀H₁₇N₂O₁₃P₃ (free acid)

Molecular Weight:
ddATP: 475.18 g/mol (free acid)
ddCTP: 451.16 g/mol (free acid)
ddGTP: 491.18 g/mol (free acid)
ddTTP: 466.17 g/mol (free acid)

Purity: ≥ 98 % (HPLC)

Form: solution in water

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: ddATP: λ_max 259 nm, ε 15.1 L mmol^-1 cm^-1 (Tris-HCl pH 7.5) ddCTP: λ_max 271 nm, ε 8.9 L mmol^-1 cm^-1 (Tris-HCl pH 7.5) ddGTP: λ_max 252 nm, ε 14.2 L mmol^-1 cm^-1 (Tris-HCl pH 7.5) ddTTP: λ_max 267 nm, ε 9.6 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Selected References:
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.