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2-Hydroxy-dATP

Isoguanosine-5'-Triphosphate, (iso-dGTP)

2-Hydroxy-2'-deoxyadenosine-5'-triphosphate, Triethylammonium salt

Cat. No. Amount Price (EUR) Buy / Note
NU-1209S 10 μl (100 mM) 98,84 Add to Basket/Quote Add to Notepad
NU-1209L 5 x 10 μl (100 mM) 426,55 Add to Basket/Quote Add to Notepad
Structural formula of 2-Hydroxy-dATP (Isoguanosine-5'-Triphosphate, (iso-dGTP), 2-Hydroxy-2'-deoxyadenosine-5'-triphosphate, Triethylammonium salt)
Structural formula of 2-Hydroxy-dATP

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C10H16N5O13P3

Molecular Weight: 507.18 g/mol

Exact Mass: 507.00 g/mol

Purity: ≥ 95 % (HPLC)

Form: clear aqueous solution

Concentration: 100 mM - 110 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 292 nm, ε 10.1 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Selected References:
Xu et al. (2006) [An optimized method for construction of genomic library]. Yi Chuan. 28 (6):717.
Xia et al. (2005) Transient state kinetic studies of the MutT-catalyzed nucleoside triphosphate pyrophosphohydrolase reaction. Biochemistry 44 (46):15334.
Kamiya et al. (2004) Mutagenesis by damaged deoxyribonucleotides and its prevention by MutT-type hydrolyzing enzymes. Nucleic Acids Symp Ser (Oxf) 48:271.
Satou et al. (2003) Mutations induced by 2-hydroxy-dATP during in vitro replication with a HeLa extract. Nucleic Acids Res Suppl. 3:325.
Satou et al. (2003) Mutagenic effects of 2-hydroxy-dATP on replication in a HeLa extract: induction of substitution and deletion mutations. NNucleic Acids Res. 31 (10):2570.
Saraswat et al. (2002) Interactions of the products, 8-oxo-dGMP, dGMP, and pyrophosphate with the MutT nucleoside triphosphate pyrophosphohydrolase. Biochemistry 41 (52):15566.
Kamiya et al. (2001) Hydrolysis of oxidized nucleotides by the Escherichia coli Orf135 protein. Biochem Biophys Res Commun. 48:271.
Kamiya et al. (2000) Two DNA polymerases of Escherichia coli display distinct misinsertion specificities for 2-hydroxy-dATP during DNA synthesis. Biochemistry 39 (31):9508.
Kamiya et al. (2000) 2-Hydroxy-dATP is incorporated opposite G by Escherichia coli DNA polymerase III resulting in high mutagenicity. Nucleic Acids Res. 28 (7):1640.