8-Hydroxy-dGTP
8-Oxo-2'-deoxyguanosine-5'-triphosphate, Sodium salt
8-Hydroxy-2'-deoxyguanosine-5'-triphosphate, Sodium salt
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| NU-1117S | 30 μl (10 mM) | 138,90 | Add to Basket/Quote Add to Notepad |
| NU-1117L | 5 x 30 μl (10 mM) | 406,80 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C10H16N5O14P3 (free acid)
Molecular Weight: 523.18 g/mol (free acid)
Exact Mass: 522.99 g/mol (free acid)
CAS#: 139307-94-1
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 245 nm, ε 12.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Influence on base excision repair[1]
Influence on erroneous incorporation by DNA-polymerases[2]
Hydrolysis to 8-oxo-dGMP by E.-coli MutT[3]
Triggering cell senescence through formation of ROS[4]
BIOZ Product Citations:
Selected References:
[1] Suzuki et al. (2010) Effect of base excision repair proteins on mutagenesis by 8-oxo-7, 8-dihydroguanine (8-hydroxyguanine) paired with cytosine and adenine. DNA Repair 9:542.
[2] Katafuchi et al. (2010) Critical amino acids in human DNA polymerases η and κ involved in erroneous incorporation of oxidized nucleotides. Nucleic Acids Res. 38:859.
[3] Nakamura et al. (2010) Structural and dynamic features of the MutT protein in the recognition of nucleotides with the mutagenic 8-oxoguanine base. J. Biol. Chem. 285:444.
[4] Raia et al. (2009) Continous elimination of oxidized nucleotides is necessary to prevent rapid onset of cellular senescence. PNAS USA 106:169.
Yoshida H. et al. (2011) Increase in CpG DNA-induced inflammatory responses by DNA oxidation in macrophages and mice. Free Radic Biol Med. 51 (2):424.
Petrie et al. (2010) Deep sequencing analysis of mutations resulting from the incorporation of dNTP analogs. Nucleic Acids Res. 38 (22):8095.
Ramírez (2004) Gene of Bacillus subtilis Encodes a Functional Antimutator 8-Oxo- (dGTP/GTP)ase and Is under Dual Control of Sigma A and Sigma F RNA Polymerases. Journal of Bacteriology 186 (4):1050.
Zaccolo et al. (1999) The effect of high-frequency random mutagenesis on in vitro protein evolution: a study on TEM-1 beta-lactamase. Journal of Molecular Biology 285 (2):775.
Zaccolo et al. (1996) An Approach to Random Mutagenesis of DNA Using Mixtures of Triphosphate Derivatives of Nucleoside Analogues. Journal of Molecular Biology 255:589.
Kai et al. (2002) An oxidized nucleotide affects DNA replication through activation of protein kinases in Xenopus egg lysates. Nucl. Acids Res. 30 (2):569.
Canitrot et al. (2000) Nucleotide excision repair DNA synthesis by excess DNA polymerase beta: a potential source of genetic instability in cancer cells. FASEB J. 14 (12):1765.
Nampalli et al. (2000) Efficient synthesis of 8-oxo-dGTP: A mutagenic nucleotide. Bioorg. Med. Chem. Lett. 10 (15):1677.
Loeb et al. (1999) Lethal mutagenesis of HIV with mutagenic nucleoside analogs. Proc. Natl. Acad. Sci. USA 96:1492.
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