Digoxigenin-modified Nucleotides for DNA Labeling
Nucleotide Selector for DNA Labeling
Select a suitable nucleotide substrate for enzymatic DNA Labeling.
Table 1: Enzymatic incorporation of Digoxigenin (DIG)-modified nucleotides.
PCR: Polymerase chain reaction with Taq Polymerase; NT: Nick Translation with DNAse I / DNA Polymerase I; Primer Extension: Primer Extension with Klenow 3'-5' exo-; 3'-End Labeling: Incorporation with Terminal deoxynucleotidyl Transferase (TdT); RT:: Reverse Transcription with Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT).
1: in-house tested 2: external reference (see datasheet) n/a: no data available - : not applicable
| DNA Labeling | cDNA Labeling |
|||||
|---|---|---|---|---|---|---|
| Nucleotide | Cat. No. | PCR | NT | Primer Extension | 3' End Labeling | RT |
| dUTP | ||||||
| Digoxigenin-11-dUTP | NU-803-DIGX | ✔1,2 | ✔1,2 | ✔2 | ✔2 | ✔2 |
| ddUTP | ||||||
| Digoxigenin-11-ddUTP | NU-1619-DIGX | - | - | - | ✔2 | - |
| dCTP | ||||||
| Digoxigenin-11-dCTP | NU-809-DIGX | n/a | n/a | n/a | n/a | n/a |
| ddCTP | ||||||
| Digoxigenin-11-ddCTP | NU-1619-DIGX | - | - | - | n/a | - |
| ddCTP | ||||||
| Digoxigenin-11-ddGTP | NU-1619-DIGX | - | - | - | n/a | - |
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Dr. Barbara Zschoernig
For inquiries regarding Nucleic Acid, Click Chemistry, Probes & Epigenetics and RNA Technologies