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GTPγS

Guanosine-5'-(γ-thio)-triphosphate, Tetralithium salt

Guanosine-5'-(3-thio)-triphosphate, Guanosine-5'-(3-thiotriphosphate)

Cat. No. Amount Price (EUR) Buy / Note
NU-412-2 2 mg 73,48 Add to Basket/Quote Add to Notepad
NU-412-10 10 mg 199,46 Add to Basket/Quote Add to Notepad
NU-412-20 20 mg 360,04 Add to Basket/Quote Add to Notepad
Structural formula of GTPγS (Guanosine-5'-(γ-thio)-triphosphate, Tetralithium salt)
Structural formula of GTPγS

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 6 months after date of delivery

Molecular Formula: C10H16N5O13P3S (free acid)

Molecular Weight: 539.25 g/mol (free acid)

CAS#: 94825-44-2

Purity: ≥ 90 % (HPLC), contains < 10 % GDP

Form: solid

Spectroscopic Properties: λmax 252 nm, ε 13.7 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Functional assay for GPCRs[1, 2]
Binding assay[3]

Specific Ligands:

Heterotrimeric and monomeric G-proteins[4]

Phosphodiesterase PDE-6[5]

for P2Y-like receptor[6] and for P2Y13 receptor[7]


Please note: For reasons of stability, please make sure that the pH value of a solution of this product never drops below 7.0. This can be achieved by dissolving the nucleotide in a buffer of your choice (50 - 100 mM, pH 7 - 10). Dissolve and adjust concentration photometrically.

Selected References:
[1] Cascio et al. (2010) In vitro and in vivo pharmacological characterization of two novel selective cannabinoid CB (2) receptor inverse agonists. Pharmacological research 61:349.
[2] Obara et al. (2010) Agonist-dependent attenuation of micro-opioid receptor-mediated G-protein activation in the dorsal root ganglia of neuropathic rats. J. Neural Transmission 117:421.
[3] Cascio et al. (2010) Evidence that the plant cannabinoid cannabigerol is a highly potent alpha2-adrenoreceptor agonist and moderately potent SHT1A receptor antagonist. Brit. J. Pharmacol. 159:129.
[4] Koval et al. (2010) Europium-labeled GTP as a general nonradioactive substitute for [35S]GTPyS in high-throughput G-protein studies. Analytical Biochem. 397:202.
[5] Yamazaki et al. (2010) Mechanism for the regulation of mammalian cGMP phosphodiesterase 6. 2: Isolation and charachterization of the transducin-activated form. Mol. Cell. Biochem. 339:235.
[6] Daniele et al. (2011) Agonist-induced desensitization/resensitization of human G protein- coupled receptor 17: a functional cross-talk between purinergic and cysteinyl-leukotriene ligands. J. Pharmacol. Exp. Ther. 338 (2):559.
[7] Marteau et al. (2003) Pharmacological characterization of human P2Y13 receptor. Mol. Pharmacol. 64 (1):104.
Schmohl et al. (2012) Functional analysis of Rho GTPase activation and inhibition in a bead-based miniaturized format.Methods. Mol. Biol. 827: 271.
Martin-Galiano AJ et al. (2011) Bacterial tubulin distinct loop sequences and primitive assembly properties support its origin from a eukaryotic tubulin ancestor. J. Bio. Chem. 286 (22):19789.
Abenza et al. (2010) Aspergillus RabB Rab5 integrates acquisition of degradative identity with the long distance movement of early endosomes. Mol. Biol. Cell. 21 (15):2756.
Beuve et al. (2005) Nitric Oxide-dependent allosteric Inhibitory Role of a Second Nucleotide Binding Site in Soluble Guanylyl Cyclase. J. Biol. Chem. 280 (12):11513.
Chang et al. (2005) Nitric Oxide-dependent Allosteric Inhibitory Role of a Second Nucleotide Binding Site in Soluble Guanylyl Cyclase. J. Biol. Chem. 280 (12):11513.
Kirilenko et al. (2002) GTP-induced membrane binding and ion channel activity of annexin VI: Is annexin VI a GTP biosensor? Biophys. J. 82 (5):2737.
Shiba et al. (2002) Hypocretin stimulates [ (35)]GTP gamma S binding in Hcrtr 2-transfected cell lines and in brain homogenate. Biochem. Biophys. Res. Commun. 294 (3):615.
Aiyar et al. (1994) Human At (1) receptor is a single-copy gene - characterization in a stable cell-line. Mol. Cell. Biochem. 131 (1):75.
Ashktorab et al. (1994) Presence of GTP-binding proteins in the plasma-membrane of the Phycomyces sporangiophore. Exp. Mycol. 18 (2):139.
Schwemmle et al. (1994) The interferon-induced 67-kDa Guanylate-binding protein (HGbp1) is a GTPase that converts GTP to GMP. J. Biol. Chem. 269 (15):11299.
Noel et al. (1993) The 2.2 Å crystal structure of transducin- alpha complexed with GTP gamma S. Nature 366:654.